Easy and precise analysis of the absolute peptide content

The determination of the net peptide content poses several challenges. For example, the analysis should be conducted with as little sample as possible. Next to small samle amounts, the analysis has to be compliant with GMP guidelines, requiring greatest accuracy and precision.

This Application Note demonstrates that the absolute peptide content of lypholized peptide powder can be easily and precisely determined using the UNICUBE® trace elemental analzyer, fully compliant to USP <1057> method 7 procedure 2 and Ph. Eur. monograph 01/2008:02533 method 7B on total protein measurement procedures.